The TERTmonitor is a real-time PCR kit for the qualitative detection of c.-124C>T and c.-146C>T mutations in the TERT gene promoter (TERTp). The method, primer/probe sequences and compositions within the TERTmonitor can be advantageously used for detecting trace amounts of c.-124 C>T and c.-146 C>T mutations present in biological samples due to its high sensitivity and specificity for these mutations.
TERTmonitor was validated in DNA extracted from frozen tissues, formalin-fixed paraffin embedded (FFPE) tissue and from urine samples. Alterations detected within TERTmonitor have been described to be present in bladder carcinoma, thyroid carcinoma, skin cancer (squamous cell carcinoma, basal cell carcinomas, melanoma), central nervous system cancer, hepatocellular carcinoma, and others.
The uniqueness of TERTp mutations, due to their location in a promoter region with a high GC base pair content (>60%), preclude traditional methods using standardized conditions (conventional real-time assays or even next-generation sequencing techniques) to be used with an efficient output.
As such, TERTmonitor uses a proprietary reaction chemistry that includes a mastermix, ultra-specific primers and genotyping probes, for the detection of c.-124C>T and c.-146C>T TERTp mutations with high sensitivity and specificity by real-time PCR. This kit was validated for use in DNA extracted from frozen, FFPE tissues and urine samples. The TERTmonitor also contains the respective internal control (IC) genes as well as negative and positive DNA controls for sample validation and qualitative analysis.
Material Safety Datasheet (MSDS) - TERTmonitor_MSDS-TERTm-001-V1.1.pdf
User Handling Guide - TERTmonitor_Handling Guide.pdf
